| 朱寿彭,肖东,韩晓枫.电镜形态和DNA链断裂研究153Sm内照射诱发骨肉瘤细胞凋亡[J].中华放射医学与防护杂志,1998,18(2):89-91.Zhu Shoupeng,Xiao Dong,Han Xiaofeng.Electron microscopic morphology and DNA chain fragmentation studies on apoptosis in bone tumor cells induced by 153Sm[J].Chin J Radiol Med Prot,1998,18(2):89-91 |
| 电镜形态和DNA链断裂研究153Sm内照射诱发骨肉瘤细胞凋亡 |
| Electron microscopic morphology and DNA chain fragmentation studies on apoptosis in bone tumor cells induced by 153Sm |
| 投稿时间：1997-09-29 修订日期：1997-12-15 |
| DOI： |
| 中文关键词: 电镜形态 DNA链断裂 153Sm-EDTMP内照射 骨肉瘤细胞 凋亡 |
| 英文关键词:Electron microscopy Morphology DNA chain fragmentation 153Sm EDTMP Internal irradiation Bone tumor cells Apoptosis |
| 基金项目:国际原子能机构资助 |
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| 摘要点击次数: 3072 |
| 全文下载次数: 1966 |
| 中文摘要: |
| 目的 对153Sm-EDTMP内照射诱发骨肉瘤细胞凋亡和DNA链断裂程度进行了研究。方法 观察153Sm-EDTMP内照射不同时间, 诱发骨肉瘤细胞凋亡的电镜形态及DNA链断裂程度。结果 研究发现, 在153Sm-EDTMP内照射作用下, 可呈现核断裂, 核染质边聚, 以及膜包裹着的凋亡小体形成的骨肉瘤细胞凋亡的形态特征。并且观察到随着153Sm-EDTMP内照射作用时间的延长, 则骨肉瘤细胞的DNA链断裂程度亦随之增升。结论 153Sm-EDTMP内照射治疗时, 可诱发骨肉瘤细胞凋亡发生, 且凋亡的程度与153Sm-EDTMP内照射作用时间相关。 |
| 英文摘要: |
| Objective To study the apoptosis and fraction of DNA chain fragmentation in bone tumor cells induced by 153Sm EDTMP.Methods Apoptosis in bone tumor cells exposed to different time periods of 153Sm EDTMP internal irradiation was observed by electron microscopic morphology and DNA chain fragmentation studies.Results It was demonstrated that the bone tumor cells internally exposed to 153Sm EDTMP displayed nuclear fragmentation,margination of condensed chromatin,and formation of membrane bounded apoptotic bodies.Our study showed that the percentage of DNA chain fragmentation increases in direct proportion to the duration of internal exposure to 153Sm EDTMP.Conclusion Apoptosis induced by 153Sm EDTMP in bone tumor cells was dependent on the time of 153Sm EDTMP exposure. |
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