| 刘承勇,杨开军,任文德,等.p53和bcl-2表达与BT325细胞辐射性凋亡的关系[J].中华放射医学与防护杂志,1998,18(2):86-88.Liu Chengyong,Yang Kaijun,Ren Wende,et al.Relationship between oncogene expression and radiation induced apoptosis of BT325 cell line[J].Chin J Radiol Med Prot,1998,18(2):86-88 |
| p53和bcl-2表达与BT325细胞辐射性凋亡的关系 |
| Relationship between oncogene expression and radiation induced apoptosis of BT325 cell line |
| 投稿时间：1996-09-02 修订日期：1997-01-09 |
| DOI： |
| 中文关键词: 凋亡 辐射 多形性胶质母细胞瘤 癌基因 |
| 英文关键词:Apoptosis Radiation BT325 cell line Oncogene |
| 基金项目: |
|
| 摘要点击次数: 2580 |
| 全文下载次数: 1795 |
| 中文摘要: |
| 目的 探讨癌基因表达的变化与辐射诱导肿瘤细胞凋亡的关系。方法 采用免疫组化技术检测人多形性胶质母细胞瘤细胞系, 经X射线处理前后p53、bcl-2基因表达的变化；原位杂交方法检测p53基因在mRNA水平表达的变化。结果 ①凋亡组内p53蛋白表达率明显高于对照组, bcl-2表达显着减弱, p53与bcl-2蛋白表达呈显着性负相关。②凋亡组内p53在mRNA水平表达量明显高于对照组, p53基因在蛋白水平与mRNA水平表达呈显着性正相关。结论 p53和bcl-2基因在X射线诱导的细胞凋亡过程中具有重要作用。 |
| 英文摘要: |
| Objective To investigate the relationship between mechanism of radiation induced apoptosis and the expresion of p53,bcl-2 genes.Methods Immunohistological staining assay (ABC assay) was used to detect the changes of p53,bcl-2 after radiation.p53 mRNA was examined by in situ hybridization in BT325 cell line after low dose radiation.Results ①Overexpression of p53 was detected in apoptotic groups (vs.control: P <0.01).Expression of bcl-2 decreased in the apoptotic group (P <0.01).The labeling index (LI) of p53 gene was significantly negatively correlated with the LI of bcl-2(0.01< P <0.05),②The LI of p53 mRNA in apoptotic groups was significantly higher than that in the control (P <0.01).Oncoprotein of p53 gene was significantly positively correlated with transcription of p53 mRNA(P <0 01).Conclusion p53 gene has promotive or inhibitory effect on radiation induced apoptosis.bcl-2 gene is an antagonist to apotosis.p53 gene and bcl-2 gene have synergetic or antagonistic relationship in the process of apoptosis. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |