| 吴玮,孔宪涛,杨如俊,李莉,闵锐,张玲珍,施笑梅,周炳荣.小鼠受亚致死剂量60Co γ 射线照射后胸腺细胞凋亡的研究[J].中华放射医学与防护杂志,1998,18(2):82-85 |
| 小鼠受亚致死剂量60Co γ 射线照射后胸腺细胞凋亡的研究 |
| Apoptosis of thymocytes in sublethally 60 Co γ irradiated mice |
| 投稿时间：1996-11-29 修订日期：1997-06-23 |
| DOI： |
| 中文关键词: 胸腺细胞 细胞凋亡 60Co γ射线 全身照射 |
| 英文关键词:Thymocytes Apoptosis Whole body irradiation |
| 基金项目:全军医药卫生科研基金 |
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| 中文摘要: |
| 目的 探讨小鼠受亚致死剂量60Coγ射线照射后胸腺细胞凋亡的变化规律, 为研究辐射后免疫功能的重建打下基础。方法 采用60Coγ射线, 2.0,4.0,6.0Gy单次全身照射, 用PI染色流式细胞仪分析, DNA琼脂糖凝胶电泳及细胞激活增殖能力的测定等方法观察胸腺细胞凋亡的规律。结果 胸腺细胞照后2小时即出现明显的细胞凋亡, 4～8小时达高峰, 后渐减少, 4.0Gy,6.0Gy在36小时内的凋亡率均比2Gy高, 照后10天三个剂量组的凋亡率基本接近正常；DNA琼脂糖凝胶电泳有大量的小分子片断, 呈典型的梯状图谱；4Gy组用PHA、rIL-2不同组合刺激受照组细胞, 发现胸腺细胞凋亡率有不同程度的下降。结论 亚致死剂量60Coγ射线照射后小鼠胸腺细胞出现明显的凋亡, 4Gy照射剂量可能是胸腺细胞达凋亡高峰的最适剂量, PHA、rIL-2联合对辐射诱导的胸腺细胞的凋亡有明显的保护作用。 |
| 英文摘要: |
| Objective The aim of this study was to investigate the apoptosis of thymocytes in 60 Coγ irradiated mice.Methods By flow cytometry,electrophoretic analysis of DNA and lymphocyte stimulating test,we determined the changes of thymocyte apoptosis in mice.The mice were whole body irradiated with 2.0,4.0 and 6.0 Gy 60 Coγ rays.Results While the thymocytes underwent apoptosis within 2 h after irradiation,fragmentation rate increased approximately 4～8 h after exposure to radiation doses of 2.0—6.0 Gy.Agarose gel electrophoresis of DNA in the 27 000×g supernatant of lysates obtained from thymocytes of irradiated mice showed ladder pattern.We found that PHA and rIL-2 prevented thymocytes from undergoing apoptosis induced by γ irradiation.Conclusion These observations suggest that the radiosensitivity of mouse thymocytes varied in different dose groups after irradiation.It is possible that PHA and rIL-2 exert protective effects on radiation iuduced apoptosis of thymocytes. |
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