| 苏旭,万虹,刘树铮.低剂量辐射对细胞凋亡相关基因蛋白表达的影响[J].中华放射医学与防护杂志,1998,18(2):77-79 |
| 低剂量辐射对细胞凋亡相关基因蛋白表达的影响 |
| Effect of low dose radiation on expression of apoptosis related gene products |
| 投稿时间：1996-06-04 修订日期：1996-10-03 |
| DOI： |
| 中文关键词: p53 bcl-2/bax 电离辐射 细胞凋亡 流式细胞术(FCM) |
| 英文关键词:Apoptosis Thymocytes Low dose radiation p53 bcl-2 bax proteins |
| 基金项目:国家自然科学基金 |
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| 摘要点击次数: 2595 |
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| 中文摘要: |
| 目的 研究低剂量辐射对细胞凋亡相关基因蛋白p53、bcl-2和bax表达的影响, 以揭示低剂量辐射免疫兴奋效应以及辐射诱导细胞凋亡“J”型曲线的分子机理。方法 采用间接免疫荧光流式细胞术。结果 75mGyX射线全身照射后24小时, p53基因蛋白产物的表达显着降低, bcl-2基因蛋白表达呈增高趋势, bax基因蛋白表达呈降低趋势, 但均无统计学意义。然而, bcl-2/bax比值则明显增加。结论 低剂量辐射全身后, p53和bcl-2/bax比值的变化表明：p53、bcl-2/bax在辐射诱导细胞凋亡的调控中起着重要作用。该结果为揭示低剂量辐射免疫兴奋效应和辐射诱导细胞凋亡“J”型曲线的分子机理提供了有意义的实验数据 |
| 英文摘要: |
| Objective To study the expression of p53,bcl-2 and bax proteins in thymocytes following low dose radiation (LDR) and to explore the molecular mechanism of radiation hormesis and dose effect “J” shape curve of apoptosis induced by radiation.Methods Kunming mice were subjected to whole body irradiation (WBI) with 75 mGy X-rays.The expression of p53,bcl-2 and bax proteins was analyzed by flow cytometry 24 hours after irradiation.Results It was found that the expression of p53 protein significantly decreased (P <0.005).The expression of bcl-2 protein showed slight but not significant increase,while expression of bax protein showed slight but not significant decrease.However,the ratio of bcl-2/bax was significantly increased (P <0.05).Conclusion The changes in expression of p53 and bcl-2/bax ratio after WBI with LDR indicate that p53 and bcl-2/bax play an important role in genic regulation on apoptosis induced by radiation.The results may provide significant data for elucidating the molecular mechanism of radiation hormesis and dose effect “J” shape curve of apoptosis induced by radiation. |
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