| 李津婴,魏善建,胡小健,张志鸿,周汉清.骨髓型急性放射病患者红细胞膜蛋白组成与功能变化特征[J].中华放射医学与防护杂志,1998,18(1):39-42 |
| 骨髓型急性放射病患者红细胞膜蛋白组成与功能变化特征 |
| Characterization of changes in composition and function of erythrocyte membrane proteins in patients with bone marrow form of acute radiation sickness |
| 投稿时间：1997-04-15 修订日期：1997-05-20 |
| DOI： |
| 中文关键词: 辐射损伤 红细胞膜蛋白 阴离子转运 |
| 英文关键词:Radiation damage Erythrocyte membrane protein Anion transport |
| 基金项目: |
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| 中文摘要: |
| 目的 观察辐射对红细胞膜损伤的远后效应。方法 应用7.5%聚丙烯酰胺凝胶电泳法、Percol梯度密度离心、NO2-通透速率测定、DIDS通透抑制率测定等方法, 分析5例骨髓型急性放射病(ARS)患者受照后第6年红细胞膜蛋白组成与功能。结果 辐射对膜蛋白的损伤持续存在, 损伤特征为带8蛋白含量减少, 带3蛋白阴离子转运功能低下。进一步分析结果显示, ARS年轻红细胞对NO-2通透时间延迟较中、老年细胞变化明显； ARS红细胞对DIDS通透抑制效应下降。结论 结果提示, 辐射对红细胞膜蛋白的损伤环节在骨髓红细胞合成期； ARS红细胞膜外侧带3结合位点可能发生病变, 导致阴离子转运功能下降； 同时, ARS红细胞可能存在提前衰老机制。 |
| 英文摘要: |
| Objective The delayed effect of radiation on erythtocyte membrane protein, the composition and function of the membrane proteins in five patients with bone marrow form of acute radiation sickness(ARS) were follow up at six years after the Shanghai 60 Co irradiation accident. Methods Percoll centrifugation, SDS polyacryamide gel electrophoresis, and analysis of NO2- transport rate and DIDS inhibition rate were performed. Results The injuries of the membrane proteins induced by radiation, characterized by reduced content of band 8 and declined anion transport function of band 3 protein remained the same as initially observed.The further study showed that the inhibition of DIDS on the anion transport of the ARS erythrocytes was decreased and the transport time for NO2- by band 3 was significantly prolonged in younger erythrocytes than those in middle or old aged cells. Conclusion It is suggested that the radiation damage to erythrocyte membrane proteins might occur at the stage of erythropoiesis in bone marrow.The exofacial site in band 3 may be changed after radiation, which could result in the abnormalities in anion transport.It is believed that the aging of erythrocytes might be present in advanced stage of ARS. |
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