中华放射医学与防护杂志

姜涛,王春燕,陈德清,等.阳江高本底辐射地区家庭成员外周血淋巴细胞非稳定性染色体畸变的剂量效应关系初探[J].中华放射医学与防护杂志,1997,17(6):390-394.Jiang Tao,Wang Chunyan,Chen Deqing,et al.Quantitative study of unstable chromosome aberrations following life time exposure of high background radiation in China[J].Chin J Radiol Med Prot,1997,17(6):390-394

阳江高本底辐射地区家庭成员外周血淋巴细胞非稳定性染色体畸变的剂量效应关系初探

Quantitative study of unstable chromosome aberrations following life time exposure of high background radiation in China

投稿时间：1997-06-05 修订日期：1997-09-25

DOI：

中文关键词: 高本底辐射家庭成员染色体畸变定量关系分析

英文关键词:High background ionizing radiation Family member Chromosome aberrations Quantitative analysis

基金项目:

作者	单位
姜涛	100088 北京, 卫生部工业卫生实验所
王春燕	100088 北京, 卫生部工业卫生实验所
陈德清	100088 北京, 卫生部工业卫生实验所
袁镛龄	湖南省劳动卫生职业病防治研究所
魏履新	100088 北京, 卫生部工业卫生实验所
早田勇	千叶放射线医学综合研究所
森岛弥重	近畿大学
中井斌	京都体质研究会
菅原努	京都体质研究会

摘要点击次数: 3256

全文下载次数: 1944

中文摘要:

目的用改良的技术方法研究高本底辐射诱发染色体畸变剂量效应关系。方法 28名受检者选自高本底和对照地区10个家庭中的祖、父、子三代。个体累积剂量分别为25.2～244.8和5.4～51.7mGy.分离淋巴细胞培养法制备染色体标本。每例分析2000～3000细胞。结果高本底地区家庭成员非稳定性染色体畸变率随年龄增长而增高(Y=0.6323+0.0463X,r=0.8750);并且其中年组和老年组平均畸变率显着高于对照组(2.56vs.1.25,P<0.05和3.60vs.1.22,P<0.001).在对照地区未见到染色体畸变率与年龄增长相关。高本底地区个体畸变率和累积剂量间呈明显的直线相关(Y=0.7441+0.0154D,r=0.86).结论高本底持续照射诱发的人体内非稳定性染色体畸变可随受照射时间的延长而持续升高,但当累积剂量降至约50mGy时,用目前方法进行定量分析是有困难的。

英文摘要:

Objective To obtain a quantitative data regarding high background radiation induced human chromosome aberrations by using advanced techniques.Methods Environmental exposure dose for each individual was carefully measured.The estimated life time doses ranged 25.2-244.8 and 5.4-51.7mGy for individuals from the high background radiation area (HBRA) and the control area,respectively.Peripheral blood specimens were taken from 28 family members of three different generations in both areas.Purified lymphocyte culture technique for chromosome preparation was adopted and a total number of 70000 metaphases were scored.Results In the case of HBRA,the frequencies of Dic+Rc increased with age,but no age dependency was observed in subjects from the control area.The mean aberration yields significantly in excess of control value were seen in two older age groups.The aberration frequencies increased in proportion to the cumulative dose of the individuals living in HBRA.The estimated rate of increase per mGy was 1.5×10^-5 per cell.Conclusion It seems that Dic can continuously accumulated over life time chronic low dose exposure and can serve as a reliable biological indicator.When the dose reduces to about 50mGy, however,it becomes difficult to use the current method for quantitative analysis.

HTML 查看全文查看/发表评论下载PDF阅读器

关闭