| 王冰,渡边惠子,山田武,等.有机氚化物对离体培养小鼠胎儿中脑细胞增殖及分化的影响[J].中华放射医学与防护杂志,1997,17(5):319-323.Wang Bing,Keiko Watanabe,Takeshi Yamada,et al.Effects of organically bound tritium (obt) on cultured midbrain cells from embryonic mice[J].Chin J Radiol Med Prot,1997,17(5):319-323 |
| 有机氚化物对离体培养小鼠胎儿中脑细胞增殖及分化的影响 |
| Effects of organically bound tritium (obt) on cultured midbrain cells from embryonic mice |
| 投稿时间：1996-03-04 修订日期：1997-07-09 |
| DOI： |
| 中文关键词: 有机氚化物 胎儿中脑细胞 相对生物效能 小鼠 |
| 英文关键词:Organically bound tritium Embryos Midbrain cells Differentiation Relative biological effectiveness Mice |
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| 中文摘要: |
| 目的 作者就四种有机氚化物对体外培养细胞的增殖及分化的影响进行了研究。方法 细胞取自11日胎龄小鼠中脑组织,对其进行高密度细胞培养,并加入有机氚化物与培养液。结果 中脑细胞的分化比增殖对辐射更敏感。辐射引起剂量依赖性的培养细胞DNA和蛋白质含量的减少。以各指标50%的抑制剂量,用X射线为参考辐射估算的氚RBE值在4.6～8.7范围。混合辐射,特别是当使用3H-TdR时,能更有效地抑制中脑细胞的分化。0.05Gy即可产生可检测到的抑制效应。结论 高密度中脑细胞培养系统对辐射极为敏感,来自有机氚化物的β射线对该系统中细胞的增殖及分化的抑制作用与参考辐射X射线相比较,具有极高的相对生物效应比值。 |
| 英文摘要: |
| Objective Four kinds of organically bound tritium compounds (OBTs) including 3H-thymidine, 3H-uridine, 3H-ariginine and 3H-glutamic acid,were investigated on proliferation and differentiation of cultured mouse embryonic midbrain cells (MBCs).Methods MBCs were isolated from day 11 embryos,cultured at a high concentration with the medium containing OBT.Results Differentiation of MBC was more sensitive to radiation than proliferation.Dose dependent decrease of DNA and protein contents were also observed.The RBE values,ranging from 4.6 to 8.7,of β rays from OBTs were obtained when compared with X irradiation at their ID50s (inhibitory dose that reduced assessment value by 50% of the control) on inhibition of cell proliferation and differentiation,and on reduction of DNA and protein contents of the cultures.The mixed exposure to X rays and one kind of OBTs or to any two kinds of OBTs resulted in more efficiently inhibitory effect on differentiation.Conclusions MBC culture system was more sensitive to beta radiation from OBTs than to X-rays,which resulted in very high RBE values. |
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