| 王兆钺,王山喜,江家贵,等.60Coγ射线分次照射对犬血浆和红细胞膜8-表氧-PGF2α含量的影响[J].中华放射医学与防护杂志,1997,17(5):312-315.Wang Zhaoyue,Wang Shanxi,Jiang Jiagui,et al.Effect of fractionated 60Coγ-ray irradiation on 8-epi-prostaglandin F2α in plasma and RBC membrane of dogs.[J].Chin J Radiol Med Prot,1997,17(5):312-315 |
| 60Coγ射线分次照射对犬血浆和红细胞膜8-表氧-PGF2α含量的影响 |
| Effect of fractionated 60Coγ-ray irradiation on 8-epi-prostaglandin F2α in plasma and RBC membrane of dogs. |
| 投稿时间：1996-01-15 修订日期：1996-04-08 |
| DOI： |
| 中文关键词: 放射损伤 8-表氧-前列腺素F2α 血浆 丙二醛 红细胞膜 |
| 英文关键词:Irradiation injury 8-epi-prostaglandin F2α Plasma Malondialdehyde RBC membrane |
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| 中文摘要: |
| 目的 观察犬放射损伤时血浆与红细胞膜8-表氧-前列腺素F2α(8-表氧-PGF2α)含量的改变,并与丙二醛(MDA)比较,探讨测定8-表氧-PGF2α对了解脂质过氧化反应的价值以及SOD对其的影响。方法 用60Coγ射线照射18只成年杂种犬,实验分低剂量SOD组,高剂量SOD组及对照组,每组6只,吸收剂量率为0.6Gy/d,共5天,计3.0Gy.测定照射前、照射过程中及照射后血浆与红细胞膜8-表氧-PGF2α及MDA含量。结果 在60Coγ射线照射期间,犬血浆8-表氧-PGF2α含量显着升高,结束照射后逐渐下降,但红细胞膜含量增加幅度更大,并于照射结束后第2天达高峰,然后开始下降；SOD对其升高有显着的抑制作用。血浆与红细胞膜MDA含量均无显着性改变。结论 8-表氧-PGF2α可作为观察放射损伤时脂质过氧化反应的一个新的较敏感的指标。 |
| 英文摘要: |
| Objective By observing the alteration of 8 epi prostaglandin F2α (8-epi-PGF2α ) in plasma and RBC membrane of dogs during and after γ irradiation, and comparing it with malondialdehyde (MDA), we aimed to assess the value of measuring its concentrations in realizing the irradiation induced lipid peroxidative reaction, and to detect the effect of superoxide dismutase (SOD) on this procedure. Methods 18 adult outbred dogs (6 dogs each were randomly assigned to low dose SOD treatment group, high dose SOD treatment group and control group) were irradiated with 60Coγ-rays at a dose of 0.6Gy per day for 5 days, and the concentrations of 8-epi-PGF2α and MDA in plasma and RBC membrane were assayed before, during, and after the irradiation. Results The mean plasma 8-epi-PGF2α level in dogs was significantly increased during the γ irradiation, and gradually decreased after the irradiation was ended, while the enhanced 8-epi-PGF2α concentration in RBC membrane was higher than that in plasma, and it reached the peak level at the 2nd day after the irradiation was ended and then started to decrease; SOD could markedly inhibit its increase. The levels of MDA in plasma and RBC membrane were not significantly changed. Conclusion 8-PGF2α can serve as a new and more sensitive index for reflecting the irradiation induced lipid peroxidative reaction. |
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