| 吴玮,孔宪涛,李莉,张玲珍,施笑梅,杨如俊,闵锐.亚致死剂量γ射线照射后小鼠脾脏CD95阳性淋巴细胞变化规律的研究[J].中华放射医学与防护杂志,1997,17(5):305-308 |
| 亚致死剂量γ射线照射后小鼠脾脏CD95阳性淋巴细胞变化规律的研究 |
| Characterization of expression of CD95 of splenic lymphocytes in sublethally γ irradiated mice |
| 投稿时间：1996-12-11 修订日期：1997-04-02 |
| DOI： |
| 中文关键词: 脾脏细胞 细胞凋亡 CD95 γ射线照射 |
| 英文关键词:Splenic lymphocytes Apoptosis CD95 Whole body irradiation |
| 基金项目:全军医学卫生科研基金 |
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| 中文摘要: |
| 目的 观察γ射线照射后小鼠脾脏淋巴细胞凋亡的变化规律与CD95(Fas)表达的关系。方法 采用PI染色及双荧光流式细胞术研究不同剂量γ射线照射后不同时间,小鼠脾脏淋巴细胞凋亡及CD95表达的规律。结果 小鼠脾脏淋巴细胞对不同剂量照射的凋亡敏感性差异有显着性,4Gy照射后凋亡率最高(在2～6Gy之间观察),淋巴细胞表面Fas的表达随照射后细胞凋亡率的变化而改变。结论 脾脏淋巴细胞在辐射后表现出明显的Fas介导的凋亡,B细胞较T细胞具有更高的辐射凋亡敏感性。 |
| 英文摘要: |
| Objective The aim of this study was to investigate the apoptosis of splenic lymphocytes and CD95 expression in 60Co γ irradiated mice. Methods Mice were whole body irradiated with 2.0, 4.0, 6.0 Gy γ-rays. The splenic lymphocytes were analyzed by flow cytometry with fluorecscent monoclonal antibodies and PI in different times after irradiation. Results It was found that apoptosis occurred in the normal splenic tissues, Fas was expressed in resting mouse splenic lymphocytes and the expression varied in T cells and B cells. B cells expressed high levels of Fas than T cells. Within 2 hours after 4Gy irradiation, however, B cells and T cells upregulated Fas expression and thereafter downregulated the expression and died by apoptosis if Fas was crosslinked. Conclusions These observations suggest that the radiosensitivity varies with different doses of irradiation in mouse splenic lymphocytes. Fas mediated apoptosis plays a major role after irradiation of aplenic lymphocytes. |
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