| 蒋伟,罗成基.TNF-α对放射所致造血损伤的保护效应[J].中华放射医学与防护杂志,1997,17(4):233-235 |
| TNF-α对放射所致造血损伤的保护效应 |
| Protective effect of TNF-α on hematopoietic impairment after radiation |
| 投稿时间：1996-05-28 修订日期：1996-10-03 |
| DOI： |
| 中文关键词: 肿瘤坏死因子 辐射防护 造血干细胞 造血祖细胞 |
| 英文关键词:TNF-α Radioprotection Hematopoietic stem cells Hematopoietic progenitor cells |
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| 中文摘要: |
| 目的 研究TNF-α对放射所致造血损伤的保护效应及其机理。方法 采用骨髓粒-单系祖细胞体外琼脂培养技术, 研究TNF-α对致死剂量60Coγ射线全身照射小鼠的防护效果；对亚致死剂量照射后小鼠造血重建的作用, 采用流式细胞技术, 分析TNF-α对小鼠骨髓细胞周期分布的影响。结果 TNF-α照射前给药能提高受致死剂量照射小鼠30天存活率至46.67%,照射后13～19天外周血白细胞、第3天骨髓有核细胞、造血干细胞以及第3,5天粒-单系祖细胞均显着高于照射对照组(P<0.01),同时发现TNF-α能阻止静止期骨髓细胞进入增殖周期。结论 肿瘤坏死因子促进照射后小鼠骨髓造血的恢复, 可能是TNF-α抗放作用的重要机理之一。 |
| 英文摘要: |
| bjective To investigate the protective effect of TNF-α on hematopoietic impairment after radiation. Methods The radioprotective effect of TNF-α on Kunming mice whole body irradiated with lethal dose of 8.5Gy gamma rays was investigated. The effects of TNF-α on hematopoietic reconstitution of mice irradiated with sublethal dose of 7.0Gy gamma rays was studied with semisolid agar colony assay of CFU-GM. Using flow cytometry, the effect of TNF-α on the distribution of cell cycle of bone marrow in mice was analyzed. Results It was found that TNF-α could effectively raise 30 day survival rate after 8.5Gy irradiation and promote the recovery of WBC, BMC, CFU-GM and CFU-S when it was given before irradiation. Additionally, we found TNF-α could prevent the static hematopoietic progenitor populations from entering proliferative state. Conclusion TNF-α can promote hematopoietic impairment after radiation, which is an important mechanism of radioprotection. |
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