| 叶治家,董燕麟,罗成基,曹廷兵,曾昭铸,程天民.放烧复合伤后白细胞介素-3基因的表达[J].中华放射医学与防护杂志,1997,17(4):230-232 |
| 放烧复合伤后白细胞介素-3基因的表达 |
| Changes of interleukin-3 expression after combined radiation-burn injury in mice |
| 投稿时间：1995-07-25 修订日期：1996-04-10 |
| DOI： |
| 中文关键词: 放射损伤 烧伤 放烧复合伤 白细胞介素-3 基因表达 |
| 英文关键词:Radiation injury Burn Combined radiation burn injury Interleukin-3 Gene |
| 基金项目:国家自然科学基金 |
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| 摘要点击次数: 2730 |
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| 中文摘要: |
| 目的 为了进一步探讨放射损伤和放烧复合伤引起造血功能障碍的机理, 研究了作为造血调控的重要细胞因子--白细胞介素-3(IL-3)基因在放射损伤、烧伤、放烧复合伤后不同时相点基因表达的变化。方法 mRNA抽提、分子杂交、细胞培养、IL-3生物活性测定、3H-TdR参与实验等生物技术方法。结果 放射损伤后IL-3基因的表达受到明显抑制, 照射后第3,14天表达的IL-3mRNA减少了77%,21%;IL-3蛋白减少了83%,36%.烧伤对IL-3基因表达的影响呈双相性, 烧伤后第3天IL-3表达受到抑制；烧伤后第14天IL-3的表达增强；放烧复合伤后IL-3的表达受到抑制, 其抑制程度介于放射损伤与烧伤之间, 各观察组IL-3基因表达的变化与骨髓有核细胞数的变化呈平行关系。结论 放射损伤、放烧复合伤后IL-3基因表达被抑制可能是急性放射病引起造血功能障碍的原因之一。 |
| 英文摘要: |
| bjective To study the changes of IL-3 expression after 5Gy irradiation, 30% total body surface area burn and combined radiation burn injury in mice. Methods Northern dot blotting, production of mRNA, cell culture, assay of IL-3 biological activity, etc. Results The IL-3 mRNA was markedly decreased by 77% and 21% on the 3rd day and the 14th day after irradiation, respectively. The IL-3 protein was decreased by 83% and 36% on 3rd and 14th day, respectively. There was a significant inhibition of IL-3 expression on the 3rd day after simple burn and slight increase of IL-3 expression on the 14th day. In groups with combined injury, IL-3 was more than that in the irradiated groups but less than that in the groups with burn injury. The changes of IL-3 expression were parallel with the changes of bone marrow cells. Conclusion Inhibition of IL-3 expression is one of the reasons of hematopoietic failure caused by radiation and combined radiation burn injury in mice. |
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