| 陈晓穗,骆训懿,周丽君,谢邦铁,王欲晓,郑辉,赵乃坤.重组人铜锌超氧化物歧化酶对小鼠抗氧化酶和免疫细胞的辐射防护作用[J].中华放射医学与防护杂志,1997,17(3):189-192 |
| 重组人铜锌超氧化物歧化酶对小鼠抗氧化酶和免疫细胞的辐射防护作用 |
| Radioprotective effect of recombinant human CuZn-SOD on activities of antioxidases and immunocytes in irradiated mice |
| 投稿时间：1996-12-11 修订日期：1997-03-25 |
| DOI： |
| 中文关键词: 辐射防护 重组人铜锌超氧化物歧化酶 抗氧化酶 免疫细胞 自由基 |
| 英文关键词:Radioprotection rhCuZn SOD Immunocytes Antioxidases Free radicals |
| 基金项目: |
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| 中文摘要: |
| 目的 探讨重组人铜锌超氧化物歧化酶(rhCuZn-SOD)的辐射防护作用。方法 观察60Coγ射线5～7Gy照射对小鼠体内抗氧化酶和免疫细胞的影响, 以及给予rhCuZn-SOD后的抗放效应。结果 与照射对照组比较, rhCuZn-SOD可明显减轻受照小鼠血液和肝细胞浆中总超氧化物歧化酶(T-SOD)、铜锌超氧化物歧化酶(CuZn-SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)的活性下降的程度。rhCuZn-SOD亦可明显增强受照小鼠脾T淋巴细胞对刀豆蛋白A的增殖反应和自然杀伤细胞的活性；提高小鼠全脾淋巴细胞、外周血白细胞和相对淋巴细胞计数；以及延长了30天平均生存时间。不同给药方法中, 辐射前后给药组的效果优于辐射前或辐射后给药组。结论 抗氧化酶活性的降低和氧自由基作用的增强可能是电离辐射引起机体过氧化损伤, 导致组织细胞功能异常的重要原因。rhCuZn-SOD对小鼠抗氧化酶和免疫细胞以及整体均具有辐射防护作用, 可能主要由于其直接清除氧自由基的功能, 其次通过这种保护功能也促进其他抗氧化酶发挥了一定的协同作用。 |
| 英文摘要: |
| Objective The radioprotective effect of recombinant human CuZn-superoxide dismutase (rhCuZn-SOD) was investigated. Method The changes of antioxidase and immunocyte activities were observed in mice given rhCuZn-SOD before and/or after 5-7 Gyγ-irradiation. Results rhCuZn SOD showed significant radioprotective effect on irradiated mice, including accelerating activities of CuZn-SOD, glutathione peroxidase (GSH-Px) and catalase (CAT) in blood and liver cells (P<0.01), reducing contents of lipid peroxidase (LPO), enhancing proliferative response of T lymphocytes to ConP<0.05), increasing the numbers of whole spleen lymphocytes, leucocytes and ralative lymphocyte counts in peripheral blood (P<0 05-0.01), and prolonging average survival time of 30 days. Conclusion Decrease of antioxidase activities and increase of free radicals may be main causes of irradiation induced peroxidation and damage to cell activities.The rhCuZn-SOD has radioprotective effect which may be due to its function of eliminating free radicals and the joint action of other antioxidases. |
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