| 陈英.辐射诱发白血病照后早期种系差异的细胞遗传学研究[J].中华放射医学与防护杂志,1997,17(3):173-177 |
| 辐射诱发白血病照后早期种系差异的细胞遗传学研究 |
| Cytogenetic analysis of strain difference during early periods after leukemogenic irradiation |
| 投稿时间：1996-12-09 修订日期：1996-12-25 |
| DOI： |
| 中文关键词: 辐射 淋巴瘤 染色体畸变 |
| 英文关键词:Irradiation Lymphoma Chromosome aberrations |
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| 中文摘要: |
| 目的 通过比较B10.Thy1.1和STS/A两种小鼠在受照后早期的染色体核型变化, 探讨特异性染色体畸变与白血病发生之间的关系。方法 选择上述两个不同种系小鼠进行照射, 于照后12～33天分次取胸腺细胞制备染色体G带标本。结果 染色体数目及结构畸变均以B10.Thy1.1小鼠明显高于STS/A小鼠。15三体的检出率在B10.Thy1.1种系中平均为37.5%, 而在STS/A种系中未见一例。在以易位为主的结构畸变中, 多数涉及12号染色体, 且主要位于E-F区。将受照小鼠胸腺移植后, 观察受体小鼠淋巴瘤的染色体核型, 发现61.5%的核型与供体的完全一致, 多数伴有15三体及12号染色体易位。结论 以15三体和12号染色体易位为代表的基因不稳定性表现在辐射诱发小鼠胸腺瘤白血病的恶性转化过程中起着至关重要的作用。 |
| 英文摘要: |
| Objectives To study the relationships between genomic instability and leukemia lymphoma development, chromosome karyotypes of B10.Thy1.1 and STS/A mice at early periods after irradiation were analyzed. Methods G-banded chromosome karyotypes of B10.Thy 1.1 and STS/A strains from thymocytes of 12 to 33 days after leukemogenic fractionated whole-body X-irradiation were compared.Results Chromosome numerical and structural aberrations in B10.Thy1.1 mice were higher than those in STS/A mice.The average incidence of trisomy 15 was 37.5% from 12-32 days after irradiation in B10.Thy1.1 mice, but none was found in STS/A mice.Various kinds of chromosome translocations were observed and most frequent translocations occurred in E F region of chromosome 12.After thymocyte transplantation, 61.5% of lymphoma karyotypes in recipient mice were the same as in irradiated donor mice, most of them having trisomy 15 and translocations of chromosome 12. Conclusions Genetic instability expressed mainly as trisomy 15 and translocations of chromosome 12 plays an important role in neoplastic transformation of mouse lymphocytes. |
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