| 杨梅英,叶常青,吴德昌,刘雷华.α 粒子诱发人胎气管永生化纤维细胞恶性转化相关基因的分析[J].中华放射医学与防护杂志,1997,17(3):166-168 |
| α 粒子诱发人胎气管永生化纤维细胞恶性转化相关基因的分析 |
| Relative gene in immortalized human fetal tracheal fibroblasts malignantly transformed by alpha particle irradiation |
| 投稿时间：1995-09-02 修订日期：1996-11-19 |
| DOI： |
| 中文关键词: α粒子照射 人胎气管永生化纤维细胞 相关基因 差异显示 |
| 英文关键词:Alpha particles Immortalized human fetal tracheal fibroblasts Relative gene |
| 基金项目:国家自然科学基金 |
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| 中文摘要: |
| 目的 探索辐射诱发细胞恶性转化相关基因。方法 本实验利用3'端引物HT11M(G, C, A)与5'端引物HAP17～24所配的24对引物同时对SHTF(SV40 immortalized humanfetal tracheal fibroblast SHTF)细胞和经238Puα粒子照射后能在软琼脂上形成克隆的此细胞(αSHTF)的mRNA进行反转录PCR扩增, 6%测序胶电泳, 放射自显影。结果 发现有23条基因片段在αSHTF细胞中表达, SHTF细胞中不表达。回收差异的基因片段, 随机取其中12条重新扩增后得到6条扩增片段。其中C17-5, C23-1两片段经Northerndot杂交后, 均与αSHTF细胞RNA有强阳性杂交信号, 与SHTF细胞RNA无杂交信号。结论 本实验证实了SHTF和αSHTF细胞的基因表达有差异存在。 |
| 英文摘要: |
| Objectives To explore the relative novel genes which are associated with radiation-induced neoplastic transformation of cells. Mothods The experiment was carried out by reverse transcription of mRNA from SHTF and α SHTF cells, amplification of cDNA by PCR using 24 pairs of primers consisting of 3'[HT11M(G, C, A)] and arbitrary 5' (HAP17～24) primers, tracing with 32P-dATP, running on 6% sequencing gels in adjacent lanes, and then autoradiographing. Results Twenty three DNA segments showed expression in α SHTF cells, but not in SHTF cells. By reamplification of 12 differential expression DNA segments of them at random, 6 DNA segments were obtained.Northern dots showed that C17 5 and C23-1 had positive responses in α SHTF cells, but negative in SHTF cells. Conclusions The research indicates that there is difference in gene expression between SHTF cells and SHTF cells. |
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