| 苏旭,张迎春,万虹,刘树铮.低剂量辐射对小鼠胸腺细胞成熟、分化、凋亡和激活的影响[J].中华放射医学与防护杂志,1997,17(3):162-165 |
| 低剂量辐射对小鼠胸腺细胞成熟、分化、凋亡和激活的影响 |
| Effects of low dose radiation on differentiation, activation and apoptosis of thymocytes in mice |
| 投稿时间：1996-12-30 修订日期：1997-01-04 |
| DOI： |
| 中文关键词: 低剂量辐射 免疫增强 细胞机理 胸腺细胞 流式细胞术 |
| 英文关键词:Low dose radiation Immunoenhancement Cellular mechanism Thymocytes Flow cytometry |
| 基金项目:国家自然科学基金 |
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| 中文摘要: |
| 目的 研究低剂量辐射对胸腺细胞成熟、分化、激活和细胞凋亡及有关基因蛋白表达的影响, 以揭示低剂量辐射免疫增强效应的发生机理。方法 采用双参数直接免疫荧光和间接免疫荧光流式细胞术, 检测了低剂量辐射小鼠全身照射后胸腺细胞CD4、CD8、TCR、CD3、IL-2R、[Ca2-]i、Bcl-2、Bax的表达和细胞凋亡。结果 实验结果表明:低剂量辐射全身照射未引起TS减少和TH/TS比值上调；低剂量辐射未增加胸腺细胞凋亡, 这与Bcl-2/Bax比值上调有关；低剂量辐射促进了胸腺细胞的成熟分化和信息传递过程。结论 以上结果提示:低剂量辐射促进胸腺细胞的成熟、分化和激活, 使胸腺向外周输送成熟的具有功能活性的效应性T细胞的储备增强, 可能是低剂量辐射免疫增强效应的重要机理。 |
| 英文摘要: |
| Objective To elucidate the possible mechanism of immunoenhancement after low dose radiation (LDR), the differentiation, activation and apoptosis of thymocytes following low dose irradiation were studied. Method Kunming mice were whole body irradiated (WBI) with low dose X-rays.The expressions of CD4, CD8, TCR, CD3, IL 2R, (Ca2+) i, Bcl-2 Bax and apoptosis of thymocytes were analyzed by flow cytometry.Results It was found that the ratio of TH/TS showed no significant changes after LDR.Thymocyte apoptosis was not increased after LDR.The increase of Bcl-2/Bax ratio after LDR might be one of its mechanisms.LDR could expedites the process of differentiation of and facilitate the signal transduction in, thymocytes. Conclusion The results indicate that the mechanism of immunoenhancement might be related to the expedition of the maturation, differentiation and activation of thymocytes, thus up-regulating the capability of supplying more mature T lymphocytes to the periphery by the thymus after LDR. |
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