| 裴雪涛,王立生,徐黎,等.脐带血造血干细胞的“质”“量”研究[J].中华放射医学与防护杂志,1997,17(3):146-150.Pei Xuetao,Wang Lisheng,Xu Li,et al.Qualitative and quantitative studies of human umbilial cord blood hematopoietic stem cells[J].Chin J Radiol Med Prot,1997,17(3):146-150 |
| 脐带血造血干细胞的“质”“量”研究 |
| Qualitative and quantitative studies of human umbilial cord blood hematopoietic stem cells |
| 投稿时间：1996-11-25 修订日期：1996-12-11 |
| DOI： |
| 中文关键词: 脐带血 造血干细胞 增殖分化 扩增 移植效率 |
| 英文关键词:Human umbilial cord blood Hematopoietic stem cells Proliferation and differentiation Expansion Grafting efficiency |
| 基金项目:国家863计划项目基金 |
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| 摘要点击次数: 3605 |
| 全文下载次数: 2015 |
| 中文摘要: |
| 目的 对脐带血造血干细胞移植的“质”“量”作出评估, 使其更广泛有效地应用于临床。方法 利用免疫磁珠分离法、FACS分析与分选、体外液体培养、铺展贴壁(Panning)等方法对脐带血CD34+造血干、祖细胞及其的数量、体外增殖分化性能、生长因子扩增效应、植入成人骨髓基质效率等进行研究, 数据经t检验。结果 脐带血有核细胞、CFCs、CD34+细胞及其亚群(CD34+CD38+和CD34+CD38-)等的绝对数量明显低于常规骨髓移植所需的细胞数量, 但脐带血CD34+CD38-亚群占CD34+细胞的比例(9.5%)明显高于骨髓或外周血(4%)(P<0.01).脐带血CD34+细胞及其亚群的增殖分化能力明显高于骨髓和外周血, 可形成更多的各系造血集落和维持更长久的造血活动。且对造血生长因子具有良好的扩增效应, 细胞总数、CFCs及CD34+细胞可分别被扩增500.0±82.5倍、94.1±28.6倍和24.0±3.8倍, 但扩增也加速了细胞的分化。此外, 仅有36%的脐带血CD34+细胞可在短时间内(2小时)植入成人骨髓基质, 但经GM-CSF和/或IL-3预处理6～8小时后, 可明显提高其移植效率, 植入成人骨髓基质的CD34+细胞达细胞达68.0%～89.6%(P<0.01).结论 通过扩增脐带血造血干祖细胞及改善其移植效率, 可使脐带血在急性放射病、造血干细胞移植、基因治疗等方面得到更广泛的应用。 |
| 英文摘要: |
| Objective To evaluate the quality and quantity of human umbilial cord blood hematopoietic stem cells for transplantation, the percentage, abilities of proliferation and differentiation, expansion responses and grafting efficiency of CD34+ cells and their subpopulations from umbilical cord blood were studied.Methods CD34+ cells and their subsets (CD34+CD38+ and CD34+CD38-) were isolated from human umbilical cord blood by using MACS or FACS system, cultured and expanded with hematopoietic growth factors (HGFs) in semi solid and liquid culture systems, and the grafting efficiency was evaluated by Panning assay. Results The percentage of CD34+CD38- subset in cord blood was much higher (9 5%) than that in bone marrow or peripheral blood (4%) (P<0.01 ). More hematopoietic colony formation from cord blood than that from bone marrow or peripheral blood was observed. With addition of HGFs, the total uncleated cells increased by 500.0±82.5-fold, the hematopoietic progenitor cells by 94.1±28.6-fold and the CD34+ cells by 24.0±3.8-fold. Only 36% of CD34+ cells were taken in a pre formed adult bone marrow stroma in a short time (2hr), but 68%-89.6% of those pretreated with GM CSF and / or IL 3 for 6-8hr were taken in the stroma (P<0.01 ). Conclusions It is possible to expand cord blood CD34+ cells ex vivo to improve their grafting efficiency for utilization of cord blood stem cells in treatment of acute radiation sickness, transplantation and therapeutic gene transfer. |
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