| 龚诒芬,郭仁锋,朱茂祥,等.吞噬细胞释放的氧自由基和肿瘤坏死因子增加α粒子致细胞转化频率的研究[J].中华放射医学与防护杂志,1997,17(2):98-101.GONG Yifen,GUO Renfeng,ZHU Maoxiang,et al.Enhancement of alpha particles induced cell transformation by oxygen free radicals and tumor necrosis factor released from phagocytes[J].Chin J Radiol Med Prot,1997,17(2):98-101 |
| 吞噬细胞释放的氧自由基和肿瘤坏死因子增加α粒子致细胞转化频率的研究 |
| Enhancement of alpha particles induced cell transformation by oxygen free radicals and tumor necrosis factor released from phagocytes |
| 投稿时间：1995-10-10 修订日期：1996-05-06 |
| DOI： |
| 中文关键词: 细胞转化 α粒子 氧自由基 肿瘤坏死因子 吞噬细胞 |
| 英文关键词:Cell Transformation Alpha particles Oxygen free radicals Tumor necrosis factor Phagocytes |
| 基金项目:本课题获国家自然科学基金（No39170273）资助 |
| 作者 | 单位 | | 龚诒芬 | 100850 北京, 北京放射医学研究所 | | 郭仁锋 | 100850 北京, 北京放射医学研究所 | | 朱茂祥 | 100850 北京, 北京放射医学研究所 | | 寿江 | 100850 北京, 北京放射医学研究所 | | L.Hieber | GSF Institute of Radiobiology, Neuherberg D-85758, Germany | | K.Peters | GSF Institute of Radiobiology, Neuherberg D-85758, Germany | | C.Schippel | GSF Institute of Radiobiology, Neuherberg D-85758, Germany | | 葛桂秀 | 100850 北京, 北京放射医学研究所 | | 杨陟华 | 100850 北京, 北京放射医学研究所 |
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| 中文摘要: |
| 目的 阐明慢性炎症时吞噬细胞释出的某些内源性因子在辐射致癌中的作用。方法 以C3H10T1/2和原代SHE为靶细胞,用238Pu为体外α照射源,用转化灶形成法观察PMA及其刺激人外周血产生的过量自由基、调理酵母多糖刺激U-937细胞释放的TNF-α等炎性因子对α粒子照射细胞转化频率(TF)的增加效应。结果 PMA和它刺激的人血使0.5 Gy α粒子照射的C3H10T1/2细胞的TF分别增高2.1和2.8倍。U-937释放的TNF-α炎症因子TF增高12倍;用抗TNF-α抗体中和后,证明U-937上清中仍有其他促癌因子存在。0.5 Gy α粒子照射的SHE细胞在体外长期传代生长对hrTNF-α具有依赖性,由此得到的40代的细胞具有致瘤性。结论 慢性炎症条件下,中性粒细胞和巨噬细胞释出的过量自由基和TNF-α等因子在低剂量辐射致癌中起重要作用。 |
| 英文摘要: |
| Objective To illustrate the role of several endogenous factors released from phagocytes under chronic inflammation in radiation-induced cancer.Methods C3T10T1/2 and SHE cells were used as targets,and 238Pu alpha source was used in alpha irradiation .The enhancement of TF in alpha particles induced cell transformation by PMA stimulated human blood and zymosan stimulated U 937 cells was studied using formation of transformed foci.Results Transformation frequency （TF） of C3H10T1/2 cells exposed to alpha particles of 0.5 Gy increased 2.1 and 2.8 fold by PMA and PMA stimulated neutrophils,respectively.TF of irradiated SHE cells at a dose of 0.5 Gy increased 12 fold by the addition of the supernatant of macrophage like U 937 cell line.It was shown that TF of irradiated SHE cells at above dose increased 8 fold by the supernatant treated with anti TNF α antibody and there were unknown promoters in it.The irradiated SHE cells treated with human recombinant TNF α could be subcultured continuously in vitro.The cells at 40th passage and two lines of monoclone cells have the ability to develop malignant tumors in nude mice.Conclusion The overdose of free radicals and TNF α released from neutrophils and macrophages have played an important role in low dose radiation-induced cancer. |
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