| 宋良文,崔雪梅,高亚兵,杨瑞彪,夏国伟,王德文.放射性肺纤维化起始部位的探讨[J].中华放射医学与防护杂志,1997,17(2):84-87 |
| 放射性肺纤维化起始部位的探讨 |
| Production site of radiation-induced pulmonary fibrosis |
| 投稿时间：1996-04-01 修订日期：1996-08-08 |
| DOI： |
| 中文关键词: 放射 肺纤维化 血管紧张素Ⅱ 羟脯氨酸 胶原 |
| 英文关键词:Radiation Pulmonary fibrosis Angiotensin Ⅱ Hydroxyproline Collagens |
| 基金项目: |
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| 中文摘要: |
| 目的 探讨早期放射性肺纤维化的始动部位及其变化规律。方法 大鼠右胸部行单次照射,吸收剂量分别为0,15,30Gy,照后又分为1,3,5月组。用生化法检测肺羟脯氨酸;用偏振光法检测Ⅰ、Ⅲ型胶原;用免疫组化法检测血管紧张素Ⅱ(AⅡ)分布。结果 肺纤维化程度与照射剂量和照后时间有关;Ⅰ、Ⅲ型胶原比例随纤维化程度加大而增加;增生的胶原纤维起自细小支气管和细小动脉外膜的成纤维细胞,肺泡间隔中Ⅰ型胶原取代Ⅲ型胶原;间质成纤维细胞和巨噬细胞AⅡ免疫组化阳性。结论 照射可刺激肺间质成纤维细胞增生,Ⅰ型胶原取代Ⅲ型胶原;间质细胞中AⅡ的表达与合成可能对此过程有一定的促进作用。 |
| 英文摘要: |
| Objective Production site development and alterations of early pulmonay fibrosis were studied. Method Single irradiation was made at right thorax of rats with 0,15 and 30 Gy of γ irradiation, respectively.The rats were divided into three groups which were sacrificed 1,3,5 months post irradiation.Hydroxyproline in lungs was measured by biochemical method.Pulmonary type I and Ⅲ collagens were measured by polarization method.Distribution of angiotensin Ⅱ （AⅡ） in pulmonary tissues was displayed by immunohistochemical method. Results Extent of pulmonary fibrosis relatively increased with irradiation dose and time elapse after irradiation.Ratio of type I to type Ⅲ collagens increased with increasing fibrosis.Proliferating collagen fibers mainly came from fibroblasts of pulmonary bronchial and arteriolar adventitia,and extended into pulmonary parenchyma.Meanwhile,type I collagen substituted for type Ⅲ collagen in interstitium of pulmonary alveoli.AⅡ was positive for fibroblasts and macrophages in pulmonary interstitium. Conclusion Irradiation can stimulate fibroblasts in interstitum proliferation,and type I collagen substitutes for type Ⅲ collagen.Expression and synthesis of AⅡ in interstitium may promote the course of pulmonary fibrosis. |
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