| 陈家佩,从玉文,马平,赵艳华.吉林放射事故病人血浆造血活性观察[J].中华放射医学与防护杂志,1997,17(1):26-29 |
| 吉林放射事故病人血浆造血活性观察 |
| Observation on hemopoietic activities of plasma in an accidentally irradiated patient in Jilin |
| 投稿时间：1996-05-03 修订日期：1996-09-03 |
| DOI： |
| 中文关键词: 辐射事故病人 血浆 造血活性 |
| 英文关键词:Hemopoietic activities Plasma Radiation accident Patient |
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| 中文摘要: |
| 目的 观察病人血浆造血活性,了解其与照后造血功能变化的可能关系。方法 以MTT法测病人血浆对NFS-60、TF-1和BET-2细胞因子依赖细胞株在有或无相应细胞因子存在时的吸光度(492nm).结果 患者血清有类似GM-CSF、IL-3和EPO的刺激活性。血浆加入含rhG-CSF和EPO的培养体系中可使NFS-60和BET-2细胞增殖受抑。上述变化与病人外周血白细胞、红细胞及血红蛋白的变化有相关之处。结论 吉林辐射事故病人血浆中有粒系和红系造血抑制活性。 |
| 英文摘要: |
| Objective The hemopoietic activities in the patient's plasma were estimated and the possible relationship between these activities and the hemopoietic function after radiation was analysed,Method Using the MTT assay,the influence of patient's plasma on the proliferation of some cytokine-dependent cell lines(such as NFS-60,TF-1 and BET-2 cells)in the presence or absence of their dependent cytokines was observed.Results Some GM-CSF-like,IL-3-like and Epo like activities could be found in the plasma.Meanwhile,the inhibitory effects on the growth of NFS-60 and BET-2 cells significantly increased when the patient's plasma was added into the culture system with rhG-CSF and rhEpo.These changes were consistent with the changes of WBC,RBC and Hb in peripheral blood of the patient.Conclusion Some erythropoietic and granulopoietic inhibitory activities were found in the plasma of an accidentally irradiated patient. |
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