| 闵锐,刘本俶,程天民,等.60Co辐射事故5名受照者外周血T细胞抗原受体基因的印迹杂交分析[J].中华放射医学与防护杂志,1996,16(6):405-408.Min Rui,Liu Benti,Cheog Tianmin,et al.Blot hybridization analysis of TCR genes of T cells for five people exposed in a radiation accident[J].Chin J Radiol Med Prot,1996,16(6):405-408 |
| 60Co辐射事故5名受照者外周血T细胞抗原受体基因的印迹杂交分析 |
| Blot hybridization analysis of TCR genes of T cells for five people exposed in a radiation accident |
| 投稿时间：1995-06-06 修订日期：1996-02-28 |
| DOI： |
| 中文关键词: TCR 基因 印迹杂交 电离辐射 |
| 英文关键词:TCR gene ionizing radiation blot hybridization |
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| 中文摘要: |
| 在低熔点琼脂糖胶内制备人淋巴细胞基因组DNA, 两种限制性核苷酸内切酶对低熔点胶块内的样本DNA进行完全消化, 以人T淋巴细胞抗原受体(TCR)的α和β链cDNA为探针, 对5名4.5年前全身受60Co大剂量受照者外周血淋巴细胞基因组DNA进行印迹杂交分析。结果发现经HindⅢ内切酶消化的样本DNA, 正常对照与受照者比较, 两种cDNA探针的杂交结果无明显区别, 经EcoRⅠ内切酶消化的DNA样本, 4名受者的αcDNA探针杂交带型不同于正常对照。作者对这一结果进行了初步分析。 |
| 英文摘要: |
| Human lymphocyte total DNA was prepared in agarose plug by mixing cells with low melting agarose, and two restriction endonucleases were used for digestion of the total DNA with human a and β TCR cDNA probes. The total digested DNA from five people who were whole body exposed to 2.0-2.5 Gy ionizing radiation in an accident 4.5 years ago was hybridized by Southern blot method. The results showed that no obvious difference in hybridization bands was found between controls and the five victims when hybridizations were fulfilled in the total DNA which was digested by Hind Ⅲ restriction endonuclease with both a and β probes. However, when the total DNA was digested with restriction endonuclease EcoRⅠ and was hybridized with TCR a probe, four of the five exposed people showed a different hybridizing band pattern comparied with the controls. The results are also discussed. |
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