| 彭瑞云,王德文,李延平,熊呈琦,夏国伟,张振声,罗庆良,董波.rhGM－CSF对狗骨髓辐射损伤治疗作用的定量病理研究[J].中华放射医学与防护杂志,1996,16(6):402-404 |
| rhGM－CSF对狗骨髓辐射损伤治疗作用的定量病理研究 |
| Quantitative pathological studies on hone marrow radiation injury treated with recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) in beagle dogs |
| 投稿时间：1995-10-07 修订日期：1996-01-26 |
| DOI： |
| 中文关键词: rhGM－CSF 骨髓 辐射图像分析 病理学 |
| 英文关键词:rhGM-CSF bone marrow radiation image analysis pathology |
| 基金项目: |
|
| 摘要点击次数: 2327 |
| 全文下载次数: 1562 |
| 中文摘要: |
| 目的: 为了解细胞因子对骨髓辐射损伤的治疗作用。方法: 用60Coγ射线以5.5Gy照射14只比格狗后, 静脉注射低剂量(10μy/kg·d)及高剂量(20μg/kg·d)rhGM-CSF, 连续20天, 动物于照射后30天活杀取胸骨, 采用H·E及Ag-nor嗜银蛋白染色和图像分析等技术, 定量研究了rhGM-CSF对骨髓造血的促进作用。结果表明: 高剂量的rhGM-CSF对狗骨髓辐射损伤后造血重建有明显的促进作用, 即rhGM-CSF能使骨髓中有核细胞数显着增多；能使增殖活跃的单核系细胞亦增多, 与照射对照组比, 差异均具有统计学意义(P<0.01或P<0.05).讨论: 高剂量rhGM-CSF对狗骨髓造血功能的重建有促进作用。 |
| 英文摘要: |
| By means of nuclear organizer region associated protein (Ag-nor) Staining and image analysis, we quantitatively studide the effect of rhGM-CSF on bone marrow hemopoiesis of 14 beagle dogse irradiated with 5.5Gy 60 Coγ-rays and treated with low (10μg/kg.d) and high (20μg/kg. d) dose of rhGM-CSF for 20 days. The dogs were sacrificed in 30 days after radiation to remove sternum. The results showed that high dose of rhGM-CSF obviously improved the reconstitution of bone marrow hemopoiesis. It may increase hemopoietic cells and monocyte lineages, and promote proliferation of these cells. All of these changes were compared with those of the controls, and the results indicate that the differences were significant (P< 0.01 or< 0.05) . The authors hold that rhGM-CSF can be used to treat bone marrow radiation injury. The probable mechanism of rhGM-CSF therapy is discussed. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |