| 杨素红,项晓琼,周平坤,等.ADPRT抑制剂对人肿瘤细胞的放射增敏效应[J].中华放射医学与防护杂志,1996,16(6):385-388.Yang Suhong,Xiang Xiaoqiong,Zhou Pingkun,et al.Radiosensitization effect of ADP-ribose trans ferase inhibitor on human tumor cells[J].Chin J Radiol Med Prot,1996,16(6):385-388 |
| ADPRT抑制剂对人肿瘤细胞的放射增敏效应 |
| Radiosensitization effect of ADP-ribose trans ferase inhibitor on human tumor cells |
| 投稿时间：1995-11-06 修订日期：1996-03-18 |
| DOI： |
| 中文关键词: 3－AB 细胞放射敏感性 DNA双链断裂 脉冲电场凝胶电泳 |
| 英文关键词:Aminobenzamide cellular radiosensitivity DNA double strand break pulsed field gel electrophoresis |
| 基金项目:国家自然科学基金 |
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| 中文摘要: |
| 目的: 从细胞的克隆形成能力和细胞DNA双链断裂及修复几方面探讨了ADP-核糖基转移酶(ADPRT)的特异性抑制剂3-氨基苯甲酰胺(3-AB)对人卵巢癌细胞株HOC8的放射增敏效应。结果表明, 3-AB能降低受照细胞的克隆形成能力；照射所诱发的初始DNA双链断裂水平不受3-AB的影响, 但细胞对双链断裂的修复能力受到抑制, 表现为慢速修复水平下降, 两方面的结果呈正相关。结论: 通过脉冲电场凝胶电泳测定DNA双链断裂及其修复水平, 可以预测细胞的放射敏感性。 |
| 英文摘要: |
| Radiosensitization effect of 3-aminobenzamide( 3-AB), a specific inhibitor of ADP-ribose transferase( ADPRT), on cell colony formation and DNA double strand break (DSB), repair of human tumor cells was studied. Results showed that 3-An reduced cell survival and checked DNA DSB repair, mainly the slow phase, but did not affect initial DNA DSB level, Good correspondence between cell survival fraction and DNA DSB repair ratio indicates that DNA DSB and its repair detected by pulsed field gel electrophoresis can be used to calculate cellular radiosensitivity . |
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