| 牟颖,刘树铮.X射线对小鼠胸腺细胞凋亡的影响[J].中华放射医学与防护杂志,1996,16(5):296-298.Mu Ying.Effect of whole-body irradiation with X-rays on apoptosis of thymocytes in mice[J].Chin J Radiol Med Prot,1996,16(5):296-298 |
| X射线对小鼠胸腺细胞凋亡的影响 |
| Effect of whole-body irradiation with X-rays on apoptosis of thymocytes in mice |
| 投稿时间：1995-07-13 修订日期：1995-10-19 |
| DOI： |
| 中文关键词: 电离辐射 胸腺 细胞凋亡 |
| 英文关键词:Irradiation Thymus Apoptosis |
| 基金项目: |
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| 中文摘要: |
| 采用荧光分光光度法对裂解DNA进行定量, 并用琼脂糖凝胶电泳法定性研究X射线全身照射后小鼠胸腺细胞凋亡。结果表明, 4GyX射线照后2小时胸腺细胞凋亡开始增加, 于照后14小时达峰值, 尔后呈下降趋势。DNA裂解率在照后24小时降至14小时的50%, 这可能是内环境中巨噬细胞吞噬凋亡小体所致。在照射后14小时研究其剂量-效应关系发现, 高剂量照射使DNA裂解明显增多, 形成180bp(basepairs)左右或其整倍数的DNA断片, 电泳呈现“梯形图谱”;而低剂量辐射可使DNA裂解率降低。剂量-效应曲线呈J型。 |
| 英文摘要: |
| Radiation-induced apoptosis of thymocytes in mice was quantified by measuring the percentage of DNA fragmentation by fluorescence spectrophotometry, and qualitatively analysed by agarose electrophoresis.The results showed that DNA fragmentation of thymocytes began to increase 2 hours after exposure to 4Gy, reaching its peak value at 14 hours.After that the percentage of DNA fragmentation came down to about 50 % of the peak value at 24 hours.It was found that the apoptosis could be induced by high dose irradiation.A typical "ladder pattern" appeared on agarose gel electrophoresis after 1-4Gy irradiation.The pattern of fragmentation represents oligonucleosomes with approximately 180bp and its multiples.The dose-effect relationship of DNA fragmentation as measured by fluorescence spectrophotometry after whole-body irradiation with 0.025-4.0Gy showed a J-shape curve with significantly reduced apoptosis at low doses. |
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