| 魏汉东,瞿成奎,贺福初,吴祖泽.细胞凋亡抑制基因Bcl－2对NIH3T3细胞增殖及辐射敏感性的影响[J].中华放射医学与防护杂志,1996,16(5):290-292 |
| 细胞凋亡抑制基因Bcl－2对NIH3T3细胞增殖及辐射敏感性的影响 |
| Effects of Bcl-2 gene transfer on proliferation and irradiation sensitivity of NIH3T3 cells |
| 投稿时间：1996-04-17 修订日期：1996-06-28 |
| DOI： |
| 中文关键词: 逆转录病毒 基因转移 Bcl－2基因 辐射敏感性 细胞增殖 细胞凋亡 |
| 英文关键词:Retrovirus Gene transfer Bcl-2 gene Irradiation sensitivity Cell proliferation |
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| 中文摘要: |
| 以含Bcl-2的重组逆转录病毒转染包装细胞系PA317, 经G418抗性筛选, 获得单克隆细胞, 扩增并收集培养上清感染NIH3T3细胞, 进而完成Bcl-2基因向NIH3T3细胞的导入。后者软琼脂集落形成率与细胞增殖速率均高于对照组, 而辐射敏感性低于对照组。表明Bcl-2基因对NIH3T3细胞的增殖和辐射敏感性均具有一定的影响。 |
| 英文摘要: |
| The packaging cell line PA317 was transfected with the recombinant retrovirus vector carrying Bcl-2.After 15 days, G-418-resistant clones were isolated and expanded, and the culture supernatant was collected to infect NIH 3 T 3.Compared with the control group, the infected NIH 3 T 3 cells were higher in agarose colony forming ability and proliferation and lower in irradiation sensitivity. |
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