中华放射医学与防护杂志

赵永良,金璀珍,刘国廉,等.α粒子诱发大鼠气管上皮细胞体外恶性转化的实验研究[J].中华放射医学与防护杂志,1996,16(4):235-237.Zhao yongliang,Jin Cuizhen,Liu Guolian,et al.Neoplastic transformation of primary rat tracheal epithelial cells in culture induced by irrad iation with α-particles[J].Chin J Radiol Med Prot,1996,16(4):235-237

α粒子诱发大鼠气管上皮细胞体外恶性转化的实验研究

Neoplastic transformation of primary rat tracheal epithelial cells in culture induced by irrad iation with α-particles

投稿时间：1995-03-31 修订日期：1995-07-20

DOI：

中文关键词: ^{238^Pu} 气管上皮细胞恶性转化

英文关键词:²³⁸Pu ratstracheal cells neoplastic transformation

基金项目:

作者	单位
赵永良	100850 北京放射医学研究所
金璀珍	100850 北京放射医学研究所
刘国廉	100850 北京放射医学研究所
吴德昌	100850 北京放射医学研究所

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中文摘要:

本文作者报道了²³⁸Puα粒子照射对体外培养大鼠气管上皮(RatTrachealEpithelium, RTE)细胞的生物学效应及恶性转化作用。结果表明, 随α粒子照射剂量增加, RTE细胞存活率呈指数下降, 以γ射线照射时的D₃₇值为参比, α粒子相对生物学效应值2.54.原代RTE细胞受4Gyα粒子照射并连续传代后, 在裸鼠体内可诱发高分化鳞癌, 因而可导致RTE细胞的体外恶性转化。

英文摘要:

Serum-free cultures of primary rat tracheal epithelial (RTE) cells from specific pathogen-free Wistar rats were used to study the cytotoxic potency and tran sforming effects induced by irradiation with ²³⁸ Pu α-particles.The results showed that α-particles were substantially more effective than γ-rays.In terms of D₃₇,the RBE of α-particles was 2.54.For transformation study,primary cell cultures received single exposure to 4Gyα-particles at a dose rate of 1.26Gy.min-1.By successive passaging,passage 40 cells produced invasive squamous cell carcinomas in two out of three animals with a latent period of 4 months upon inoculation into nude mice.This culture system is a useful model to study the mechanism of radiation carcinogenesis.

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