| 史剑慧,程文英,杨星,等.原位缺口平移测定鼻咽癌细胞的放射敏感性[J].中华放射医学与防护杂志,1996,16(3):173-175.Shi Jianhui,Cheng Wenying,Yang Xing,et al.Evaluation of radiosensitivity of nasopharyngeal cancer cells by in situ nick translation[J].Chin J Radiol Med Prot,1996,16(3):173-175 |
| 原位缺口平移测定鼻咽癌细胞的放射敏感性 |
| Evaluation of radiosensitivity of nasopharyngeal cancer cells by in situ nick translation |
| 投稿时间：1995-03-21 修订日期：1995-07-14 |
| DOI： |
| 中文关键词: 原位缺口平移 鼻咽癌细胞株放射敏感性 |
| 英文关键词:In situ nick translation nasopharyngeal carcinoma cell line radiosensitivity |
| 基金项目: |
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| 摘要点击次数: 4646 |
| 全文下载次数: 1904 |
| 中文摘要: |
| 报道了以原位缺口平移(ISNT)技术研究鼻咽癌细胞株CNE、CNE-2Z对γ射线的放射效应, 以及用存活曲线验证ISNT的可靠性。结果表明, 在0～8Gy剂量之间, CNE及CNE-2Z的脱氧三磷酸核苷(dNTP)掺入率与照射剂量间呈良好量效关系；CNE-2Z的放射敏感性高于CNE.相关分析表明, cNE及CNE-2Z细胞ISNT的dNTP掺入率与存活分数之间均有良好的相关关系。表明ISNT可以较好地反映射线对肿瘤细胞的损伤效应, 可替代复杂的集落形成试验, 可望成为评价肿瘤细胞放射敏感性的一个重要指标。 |
| 英文摘要: |
| We evaluated the radiosensitivity of two nasopharyngeal cell lines which had been treated with γ-rays by in situ nick translation(ISNT).The results indicated that the incorporation rate of dNTP of thetwo cell lines increased with the radiation dose.Good correlations were found between the radiosensitivity determined by the in situ nick translation assay andconfirmed by the colony-forming assay in CNE and CNE-2Z cell lines.Although this report deals with cultured cells, the described technique of datecting DNA nickin situ without destruction of morphology seems to be applicable to tissue section.It may be possible to use it as a tool for predicting radiosensitivity of cancerous tissues treated with γ-rays. |
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