| 涂开成,曹珍山,祝宵霞,周淑珍,杨素霞,樊飞跃,刘国廉.形态计量学在60Coγ射线照射体外诱发人胚肺细胞转化研究中的应用[J].中华放射医学与防护杂志,1995,15(4):255-259 |
| 形态计量学在60Coγ射线照射体外诱发人胚肺细胞转化研究中的应用 |
| Morphometric study of malignant transformation of human embryo lung cells in vitro induced by 60Coγ-rays |
| 投稿时间：1994-01-20 修订日期：1995-02-22 |
| DOI： |
| 中文关键词: 细胞转化 形态计量 剂量率效应 人胚肺细胞 |
| 英文关键词:Human embryo lung cells transformation morphometry radiation effect dose rate |
| 基金项目:国家自然科学基金 |
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| 摘要点击次数: 2333 |
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| 中文摘要: |
| 作者应用图像分析技术,观察了60Coγ射线在两种剂量率条件下体外诱发人胚肺细胞转化的细胞和细胞核形态计量学参数的变化特点,分析了各形态计量学参数与剂量率及照射剂量两个因素间的定量关系。结果说明：人胚肺细胞的面积、等效直径和形状因子及细胞核的面积、周长、等效直径和核浆比等,均随着照射剂量的增加而增大,用线性平方模型(E=a+bD+cD2)描述这种剂量效应关系为最好；上述细胞和细胞核各参数与剂量率和照射剂量间经数学分析处理,除细胞等效直径仅与照射剂量相关外,其余参数均与上述两因素有密切的函数依赖关系,高剂量组的损伤效应明显高于低剂量率组。 |
| 英文摘要: |
| Morhpometric differences of transformed human embryo lung (HEL) cells in vitro induced by 60Coγ-rays at different dose rates were studied by image analysis. Relationship between the morphometric parameters of cells and cellular nuclei on the one hand and the dose rate and irradiation dose on the other hand was analyzed quantitatively. The results show that the area, equivalent diameter and shape factor of cell increased with increasing irradiation doses; so did the area,perimeter,eqivalent diameter of cellular nuclei and ratio of nucleus to plasma. A linear square equation (E=a+bD+c D2)can fit satisfactorily to show the the relationship between dose and effect.These morphometric parameters demonstrated close functional dependent relation to dose rate and irradiation dose, except that the equivalent diameter of cells was related to irradiation dose only. Damage effect on large doses groups was more obvious than that on small dose groups. |
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